HPLC-MS (liquid chromatography-mass spectrometry) is a sophisticated analytical instrument that combines liquid chromatography (LC) as the separation system and mass spectrometry (MS) as the detection system. LC separates samples through a chromatographic column, and the separated compounds are then detected by MS.
Instrument and Technical Parameters
- Instrument Model:Agilent 6400
- Instrument Technical Parameters
-
- Mass range: 15–2000 amu
-
- Resolution: m/z=0.1
-
- Scanning speed: 5,200 u/second
Sample Submission Requirements and Notes
- Samples can be solid or liquid, weighing 20–100 mg, stored in a plastic centrifuge tube.
- The sample must be completely soluble in a volatile common solvent (e.g., methanol, ethanol, acetone, etc.).
- The sample should have ultraviolet (UV) absorbance.
Testing Cases
Reference Standards
- ISO 21676:2018: Water quality - Determination of dissolved fractions of selected active pharmaceutical ingredients, transformation products, and other organic substances in water and treated wastewater - Methods using high-performance liquid chromatography and mass spectrometric detection (HPLC-MS/MS or HRMS) after direct injection.
- GB/T 39998-2021: Paper, board, and paper products - Determination of alkylphenol ethoxylates - High-performance liquid chromatography-mass spectrometry method.
- SN/T 4058-2014: Detection method for zearalanol residues in export animal-derived foods - Immunoaffinity column purification - HPLC and LC-MS/MS methods.
