Maldi-tof Testing - Matrix-Assisted Laser Desorption Time-of-Flight Mass Spectrometry Detection - Shanghai Jituo Technology

MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry) ionizes biomolecules via matrix-assisted laser desorption, detects their mass-to-charge ratio (m/z) using time-of-flight differences, and forms mass spectra for analysis.

Instrument Model and Technical Parameters

• Instrument Model: Bruker rapifleX
• Technical Parameters:
  • Linear mode: ≥ 1,200 (cytochrome C, m/z 12361)
  • Reflectron mode: ≥ 25,000 (cytochrome C, m/z 12361)
  • Reflectron mode: > 50,000 (for peptides)
  • Sensitivity in linear mode: 500 fmol, signal-to-noise ratio > 100:1 (BSA sample)
  • Sensitivity in reflectron mode: 250 amol, signal-to-noise ratio > 200:1 (Glu-Fib sample)
  • Linear mode:
    • Internal calibration: Mass accuracy ≤ 50 ppm (protein mixture)
    • External calibration: Mass accuracy ≤ 60 ppm (protein mixture)
  • Reflectron mode:
    • Internal calibration: Mass accuracy ≤ 1.5 ppm
    • External calibration: Mass accuracy ≤ 5 ppm

Sample Submission Requirements and Notes

• Sample form: Solid or liquid, 20–100 mg in a plastic centrifuge tube.
• The sample must be completely soluble in a volatile common solvent (e.g., methanol, ethanol, acetone, etc.).

Testing Examples

Reference Standards